Pyruvate Dehydrogenase (C-Term) anticorps

N° du produit ABIN2465266

Détail du produit

Antigène: Pyruvate Dehydrogenase

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificité: This antibody is expected to recognise all reported isoforms.

Purification: Pyruvate Dehydrogenase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Pyruvate Dehydrogenase antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Pyruvate Dehydrogenase.

Information d'application

Indications d'application: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approximately 50 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20 °C.

Détail du antigène

Antigène: Pyruvate Dehydrogenase

Synonymes: anticorps PPM2C2, anticorps pyruvate dehyrogenase phosphatase catalytic subunit 2, anticorps PDP2

ID gène: 8050

NCBI Accession: NP_003468

UniProt: O00330