Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
ACAP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
ACAP2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ACAP2.
ACAP2
Reactivité: Humain
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Alexa Fluor 350
Indications d'application
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 90 kDa band observed in human T cell line MOLT4 lysates (calculated MW of 88.1 kDa according to NP_036419). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
Centaurin beta 2 (ACAP2)
(ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 2 (ACAP2))