APPL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
APPL1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of APPL1.
APPL1
Reactivité: Humain, Souris, Rat
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 80 kDa band observed in rat Aortic Smooth muscle Cells (lane 1), intensity increased after infection with human aPPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7 kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.