Cet anticorp Cytochrome B Reductase 1 est non-conjugé
Application
ELISA, Immunohistochemistry (IHC)
Purification
Cytochrome B Reductase 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
Cytochrome B Reductase 1 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of Cytochrome B Reductase 1.
CYBRD1
Reactivité: Souris
ELISA, WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approximately 35 kDa and a 26+28 kDa doublet in human colon lysate after 0.1 μg/mL antibody staining. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration, 2-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.