Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
MDA5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
MDA5 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of MDA5.
IFIH1
Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval
WB, IHC, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:32000. Western Blot: No signal obtained yet but low background observed in human placenta lysate at up to 1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
IFIH1
(Interferon Induced with Helicase C Domain 1 (IFIH1))