SEMA3E antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
SEMA3E antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of SEMA3E.
SEMA3E
Reactivité: Humain, Souris
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Alexa Fluor 555
Indications d'application
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 35 kDa and 20 kDa in human prostate lysates after 0.1 μg/mL antibody staining.2 kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
SEMA3E
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))