Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
UNC13D antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
UNC13D antibody was raised against a 14 amino acid synthetic peptide near the internal region of UNC13D.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 105 kDa band observed in human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa according to NP_954712.1). Recommended concentration: 1-3 μg/mL. Immunohistochemisty: In paraffin embedded human thymus shows cytoplasm staining in select areas. Recommended concentration, 2.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.