Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
PGLYRP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
PGLYRP1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of PGLYRP1.
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 30 kDa band observed in Jurkat lysate (calculated MW of 21.7 kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in liter Immunohistochemistry: In paraffin embedded human spleen shows staining of select splenocytes. Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))