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HSP27 anticorps (Atto 390)

HSPB1 Reactivité: Humain WB, IHC, IF, FACS, IP, ICC Hôte: Souris Monoclonal 8A7 Atto 390
N° du produit ABIN2481411
  • Antigène Voir toutes HSP27 (HSPB1) Anticorps
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    Reactivité
    • 299
    • 104
    • 90
    • 24
    • 14
    • 14
    • 13
    • 12
    • 11
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 238
    • 65
    Souris
    Clonalité
    • 207
    • 95
    Monoclonal
    Conjugué
    • 138
    • 23
    • 17
    • 13
    • 12
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HSP27 est conjugé à/à la Atto 390
    Application
    • 256
    • 103
    • 103
    • 95
    • 69
    • 49
    • 48
    • 46
    • 41
    • 40
    • 16
    • 12
    • 10
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificité
    Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
     Réactivité croisée
    Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Rat, Mouton
    Purification
    Protein G Purified
    Immunogène
    HSP27 peptide
    Clone
    8A7
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product HSPB1 Anticorps primaire
  • Indications d'application
    • WB (1:5000)
    • ICC/IF (1:200)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:5000 dilution of ABIN2481411 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    Autre désignation
    HSP27 (HSPB1 Produits)
    Synonymes
    anticorps CMT2F, anticorps HMN2B, anticorps HS.76067, anticorps HSP27, anticorps HSP28, anticorps Hsp25, anticorps SRP27, anticorps 27kDa, anticorps Hsp27, anticorps cb153, anticorps cb660, anticorps hsp1, anticorps hsp25, anticorps hsp27, anticorps id:ibd2821, anticorps sb:cb660, anticorps zgc:103437, anticorps HSPB1, anticorps cmt2f, anticorps hs.76067, anticorps hsp28, anticorps heat shock protein family B (small) member 1, anticorps heat shock protein 1, anticorps heat shock protein, alpha-crystallin-related, 1, anticorps heat shock protein family B (small) member 1 L homeolog, anticorps HSPB1, anticorps Hspb1, anticorps hspb1, anticorps hspb1.L
    Sujet
    HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.
    ID gène
    3315
    NCBI Accession
    NP_001532
    Pathways
    Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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