TLR4 anticorps (AA 420-435) (Biotin)

N° du produit ABIN2485971

Cet anticorps Lapin Polyclonal détecte spécifiquement TLR4 dans WB, FACS, IHC, IF et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour TLR4 anticorps (AA 420-435) (Biotin) (ABIN2485971)

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TLR4 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-TLR4 anticorps (Biotin)

Épitope: AA 420-435

Specificité: Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Réactivité croisée: Humain, Souris

Purification: Protein A Purified

Immunogène: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Information d'application

Indications d'application:

• WB (1:500)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Commentaires:

2 μg/ml of ABIN2485971 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: TLR4

Sujet: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).

ID gène: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins