TNF alpha anticorps (Biotin)

N° du produit ABIN264362

L’anticorps anti-TNF alpha Monoclonal Souris est utilisé pour la détection de TNF alpha dans des échantillons de Humain et Singe. Il a été validé pour EIA.

Aperçu rapide pour TNF alpha anticorps (Biotin) (ABIN264362)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est conjugé à/à la Biotin

Application: Enzyme Immunoassay (EIA)

Clone: AS1

Détail du produit anti-TNF alpha anticorps (Biotin)

Attributs du produit: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Purification: Protein G Chromatography

Pureté: > 95 % by SDS-PAGE

Immunogène: Genename: TNF

Isotype: IgG1

Information d'application

Indications d'application: ELISA: This antibody has been used as a solution phase antibody at 2 μg/mL in a two site ELISAwith unconjugated TNFalpha antibody (Cat. No. ABIN264365). Typically the ELISA has aworking range of 0-10000 pg/mL and a sensitivity of 10 pg/mL with the appropriatestandard. Recommended sample volumes are 25-200 μL. Recovery of human TNFa in human serum is typically >85 % in the ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor (TNF-Alpha)

Sujet: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome