Granzyme B (GZMB) anticorps (Alexa Fluor 647)

Détails pour le produit réf. ABIN2657899
Antigène
  • CCPI
  • CGL-1
  • CGL1
  • CSP-B
  • CSPB
  • CTLA1
  • CTSGL1
  • HLP
  • SECT
  • granzyme B
  • GZMB
Reactivité
Humain, Souris
220
73
55
14
4
3
1
1
Hôte
Souris
141
87
3
Clonalité (Clone)
Monoclonal ()
Conjugué
Alexa Fluor 647
19
11
4
4
4
4
4
3
3
3
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
136
80
68
45
44
36
32
10
10
10
10
7
3
2
2
1
1
Options
Clone GB11
Isotype IgG1 kappa
 Réactivité croisée Rat (Rattus)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Antigène
Autre désignation Granzyme B (GZMB Antibody Extrait)
Sujet Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Protect from prolonged exposure to light. Do not freeze.
Stock 4 °C
Stockage commentaire The antibody solution should be stored undiluted between 2°C and 8°C
Images (Fournisseur)
Flow Cytometry (FACS) image for anti-Granzyme B (GZMB) antibody (Alexa Fluor 647) (ABIN2657899) anti-Granzyme B (GZMB) antibody (Alexa Fluor 647)
Flow Cytometry (FACS) image for anti-Granzyme B (GZMB) antibody (Alexa Fluor 647) (ABIN2657899) anti-Granzyme B (GZMB) antibody (Alexa Fluor 647) (Image 2)
Flow Cytometry (FACS) image for anti-Granzyme B (GZMB) antibody (Alexa Fluor 647) (ABIN2657899) anti-Granzyme B (GZMB) antibody (Alexa Fluor 647) (Image 3)
Background publications Khan, Srivastava, Spencer, Garg, Fremgen, Vahed, Lopes, Pham, Hewett, Kuang, Ong, Huang, Scarfone, Nesburn, Wechsler, BenMohamed: "Phenotypic and functional characterization of herpes simplex virus glycoprotein B epitope-specific effector and memory CD8+ T cells from symptomatic and asymptomatic individuals with ocular herpes." dans: Journal of virology, Vol. 89, Issue 7, pp. 3776-92, 2015 (PubMed).

Srivastava, Khan, Spencer, Vahed, Lopes, Thai, Wang, Pham, Huang, Scarfone, Nesburn, Wechsler, BenMohamed et al.: "HLA-A02:01-restricted epitopes identified from the herpes simplex virus tegument protein VP11/12 preferentially recall polyfunctional effector memory CD8+ T cells from seropositive asymptomatic ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 5, pp. 2232-48, 2015 (PubMed).

Wagner, Stegmaier, Hänsch: "Expression of granzyme B in peripheral blood polymorphonuclear neutrophils (PMN), myeloid cell lines and in PMN derived from haemotopoietic stem cells in vitro." dans: Molecular immunology, Vol. 45, Issue 6, pp. 1761-6, 2008 (PubMed).

Arens, Schepers, Nolte, van Oosterwijk, van Lier, Schumacher, van Oers: "Tumor rejection induced by CD70-mediated quantitative and qualitative effects on effector CD8+ T cell formation." dans: The Journal of experimental medicine, Vol. 199, Issue 11, pp. 1595-605, 2004 (PubMed).

Lima, Almeida, Dos Anjos Teixeira, Alguero Md, Santos, Balanzategui, Queirós, Bárcena, Izarra, Fonseca, Bueno, Justiça, Gonzalez, San Miguel, Orfao: "TCRalphabeta+/CD4+ large granular lymphocytosis: a new clonal T-cell lymphoproliferative disorder." dans: The American journal of pathology, Vol. 163, Issue 2, pp. 763-71, 2003 (PubMed).

Estébanez-Perpiña, Fuentes-Prior, Belorgey, Braun, Kiefersauer, Maskos, Huber, Rubin, Bode: "Crystal structure of the caspase activator human granzyme B, a proteinase highly specific for an Asp-P1 residue." dans: Biological chemistry, Vol. 381, Issue 12, pp. 1203-14, 2001 (PubMed).

Spaeny-Dekking, Hanna, Wolbink, Wever, Kummer, Kummer, Swaak, Middeldorp, Huisman, Froelich, Hack: "Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3610-6, 1998 (PubMed).

Wever, Van Der Vliet, Spaeny, Wolbink, Van Diepen, Froelich, Hack, ten Berge: "The CD8+ granzyme B+ T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells." dans: Immunology, Vol. 93, Issue 3, pp. 383-9, 1998 (PubMed).

Griffiths, Isaaz: "Granzymes A and B are targeted to the lytic granules of lymphocytes by the mannose-6-phosphate receptor." dans: The Journal of cell biology, Vol. 120, Issue 4, pp. 885-96, 1993 (PubMed).

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