5-Methylcytosine anticorps

N° du produit ABIN2668982

Détail du produit

Antigène: 5-Methylcytosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Methylated DNA Immunoprecipitation (MeDIP), Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (IHC)

Purification: Protein A Chromatography

Immunogène: Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.

Clone: 33D3

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 10 mM phosphate, 0.15 M NaCl and 0.01 % thimerosal. Thimerosal is highly toxic.

Conseil sur la manipulation:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -20 °C

Stockage commentaire: Antibodies in solution can be stored at -20 °C for 2 years.

Date de péremption: 6 months

Référence

Borghesan, Fusilli, Rappa, Panebianco, Rizzo, Oben, Mazzoccoli, Faulkes, Pata, Agodi, Rezaee, Minogue, Warren, Peterson, Sedivy, Douet, Buschbeck, Cappello, Mazza, Pazienza, Vinciguerra: "DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression." dans: Cancer research, Vol. 76, Issue 3, pp. 594-606, (2016) (PubMed).

McPherson, Fullston, Kang, Sandeman, Corbett, Owens, Lane: "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." dans: Scientific reports, Vol. 6, pp. 27010, (2016) (PubMed).

Banh, Iorio, Marcotte, Xu, Cojocari, Rahman, Pawling, Zhang, Sinha, Rose, Isasa, Zhang, Wu, Virtanen, Hitomi, Habu, Sidhu, Koizumi, Wilkins, Kislinger, Gygi, Schofield, Dennis, Wouters, Neel: "PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia." dans: Nature cell biology, Vol. 18, Issue 7, pp. 803-813, (2016) (PubMed).

Harrison, Cornett, Goldfarb, DaRosa, Li, Yan, Dickson, Guo, Cantu, Kaustov, Brown, Arrowsmith, Erie, Major, Klevit, Krajewski, Kuhlman, Strahl, Rothbart: "Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1." dans: eLife, Vol. 5, (2016) (PubMed).

Yang, Ma, Li, Zhang, Wan, Du, Li, Yang, Lu: "Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression." dans: Oncology letters, Vol. 12, Issue 4, pp. 2789-2795, (2016) (PubMed).

Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." dans: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

Xu, Wang, Long, Zhang, Wei, Xing, Gu, Xie: "Stress-mediated p38 activation promotes somatic cell reprogramming." dans: Cell research, Vol. 23, Issue 1, pp. 131-41, (2013) (PubMed).

Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." dans: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

Habib, Fares, Bourgeois, Bella, Bernardino, Hernandez-Blazquez, de Capoa, Niveleau: "DNA global hypomethylation in EBV-transformed interphase nuclei." dans: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, (1999) (PubMed).

Détail du antigène

Antigène: 5-Methylcytosine

Classe de substances: Chemical