IL4 Receptor anticorps

N° du produit ABIN2688940

Détail du produit anti-IL4 Receptor anticorps

Antigène: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL4 Receptor est non-conjugé

Application: ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Marque: BD Pharmingen™

Attributs du produit: The mIL4R-M2 monoclonal antibody specifically binds to the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα, aka, CD124). The mIL4R-M2 antibody does not recognize human or rat IL-4R, crossreactivities with IL-4R from other species have not been established. The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M2 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M2 antibody also recognizes naturally occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNA splicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. The immunogen used to generate the mIL4R-M2 hybridoma was CTLL-19.4 cells, a subclone of CTLL-2 cells that express high levels of IL-4Rα.

BD Pharmingen™ Purified Rat Anti-Mouse CD124 - Purified - Clone mIL4R-M2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: CTLL-19.4 cells

Clone: MIL4R

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IL4 Receptor (ABIN2688940)

Kubo, Yamashita, Abe, Tada, Okumura, Ransom, Nakayama: "CD28 costimulation accelerates IL-4 receptor sensitivity and IL-4-mediated Th2 differentiation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2432-42, (1999) (PubMed).

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." dans: Blood, Vol. 89, Issue 2, pp. 610-20, (1997) (PubMed).

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, (1994) (PubMed).

Sempowski, Beckmann, Derdak, Phipps: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, (1994) (PubMed).

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." dans: Blood, Vol. 78, Issue 7, pp. 1678-84, (1991) (PubMed).

Beckmann, Schooley, Gallis, Vanden Bos, Friend, Alpert, Raunio, Prickett, Baker, Park: "Monoclonal antibodies block murine IL-4 receptor function." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4212-7, (1990) (PubMed).

Mosley, Beckmann, March, Idzerda, Gimpel, VandenBos, Friend, Alpert, Anderson, Jackson: "The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms." dans: Cell, Vol. 59, Issue 2, pp. 335-48, (1989) (PubMed).

Lowenthal, Castle, Christiansen, Schreurs, Rennick, Arai, Hoy, Takebe, Howard: "Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 2, pp. 456-64, (1988) (PubMed).

Détails sur IL4 Receptor

Antigène: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Autre désignation: CD124 (IL4R Produits)

Synonymes: anticorps IL4R, anticorps il-4ra, anticorps zgc:113063, anticorps CD124, anticorps IL-4RA, anticorps IL4RA, anticorps Il4ra, anticorps IL4, anticorps Il4r, anticorps interleukin 4 receptor, anticorps interleukin 4 receptor, tandem duplicate 1, anticorps interleukin 4 receptor, alpha, anticorps IL4R, anticorps il4r.1, anticorps Il4r, anticorps Il4ra

Sujet: Synonyms: IL-4 Receptor α chain

Pathways: Signalistation JAK/STAT, Positive Regulation of Immune Effector Process