CD200 anticorps

N° du produit ABIN2689079

Détail du produit anti-CD200 anticorps

Antigène: CD200

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD200 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Marque: BD Pharmingen™

Attributs du produit: The OX-90 antibody reacts with CD200 (OX-2 antigen), a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 is expressed on thymocytes, neurons, B lymphocytes, splenic follicular dendritic cells and endothelium, and subsets of T lymphocytes and dendritic cells, but not on NK cells, granulocytes, monocytes, or macrophages. The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or down-regulation of myeloid cell activity. Multi-color analysis of the expression of CD200 on thymocytes. C57BL/6 thymocytes were stained with either purified mAb OX-90 (open histograms) or purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, shaded histograms), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) then Streptavidin-PE (Cat. No. 554061). Then the cells were stained with FITC-conjugated anti-mouse CD4 mAb and APC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553035). Electronic gates were set to include the CD4-CD8a-, CD4+CD8a+, CD4+CD8a-, and CD4-CD8a+ populations, and their expression of CD200 is displayed in the corresponding panels. Flow cytometry was performed on a BD FACSCalibur™

BD Pharmingen™ Purified Rat Anti-Mouse CD200 - Purified - Clone OX-90 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Soluble fusion protein of the extracellular region of mouse OX-2 antigen w/domains 3 & 4 of rat CD4

Clone: OX

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry System.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD200 (ABIN2689079)

Hoek, Ruuls, Murphy, Wright, Goddard, Zurawski, Blom, Homola, Streit, Brown, Barclay, Sedgwick: "Down-regulation of the macrophage lineage through interaction with OX2 (CD200)." dans: Science (New York, N.Y.), Vol. 290, Issue 5497, pp. 1768-71, (2000) (PubMed).

Gorczynski, Chen, Hu, Kai, Lei, Ramakrishna, Gorczynski: "Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 774-81, (1999) (PubMed).

Preston, Wright, Starr, Barclay, Brown: "The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages." dans: European journal of immunology, Vol. 27, Issue 8, pp. 1911-8, (1997) (PubMed).

Détails sur CD200

Antigène: CD200

Autre désignation: CD200 (CD200 Produits)

Synonymes: anticorps MOX1, anticorps MOX2, anticorps MRC, anticorps OX-2, anticorps CD200, anticorps Cspmo2, anticorps MRCOX2, anticorps Mox2, anticorps DKFZp459C0716, anticorps OX2, anticorps CD200 molecule, anticorps Cd200 molecule, anticorps CD200 antigen, anticorps CD200, anticorps Cd200

Sujet: Synonyms: OX-2 Antigen