The 28-14-8 antibody reacts with the α3 domain of the H-2D[b] MHC class I alloantigen. The antibody binds to H-2D[b] in the presence or absence of the β2 microglobulin chain. It cross-reacts with the α3 domain of H-2L[d], but not with K[d] or D[d], and with H-2D[q] and/or L[q]. Reactivity with haplotypes k, f, p, r, and s has not been observed. mAb 28-14-8 has been shown to block H-2L[d]- specific and H- 2L[d]-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes (CTL), but it does not block recognition of H-2L[d] positive target cells by Ly-6G2-positive NK cells.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Kündig, Bachmann, DiPaolo, Simard, Battegay, Lother, Gessner, Kühlcke, Ohashi, Hengartner: "Fibroblasts as efficient antigen-presenting cells in lymphoid organs." dans: Science (New York, N.Y.), Vol. 268, Issue 5215, pp. 1343-7, (1995) (PubMed).
Mason, Ortaldo, Young, Kumar, Bennett, Anderson: "Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2)" dans: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 293-303, (1995) (PubMed).
Allen, Fraser, Flyer, Calvin, Flavell: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).
Allen, Wraith, Pala, Askonas, Flavell: "Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites." dans: Nature, Vol. 309, Issue 5965, pp. 279-81, (1984) (PubMed).
Orn, Goodenow, Hood, Brayton, Woodward, Harmon, Frelinger: "Product of a transferred H-2Ld gene acts as restriction element for LCMV-specific killer T cells." dans: Nature, Vol. 297, Issue 5865, pp. 415-7, (1982) (PubMed).
Woodward, Orn, Harmon, Goodenow, Hood, Frelinger: "Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 11, pp. 3613-7, (1982) (PubMed).
Ozato, Hansen, Sachs: "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 6, pp. 2473-7, (1981) (PubMed).
Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).
Antigène
H2-D1
(Histocompatibility 2, D Region Locus 1 (H2-D1))