IL12 anticorps (Biotin)

N° du produit ABIN2689732

Cet anticorps anti-IL12 est un anticorps Souris Monoclonal détectant IL12 dans ELISA. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour IL12 anticorps (Biotin) (ABIN2689732)

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL12 est conjugé à/à la Biotin

Application: ELISA

Clone: C8-6

Détail du produit anti-IL12 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN-γ induction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23. This antibody is routinely tested by ELISA as a detection antibody. Other applications were tested during antibody development only or reported in the literature. Human IL-12 p70 Protein Quantitation by ELISA. Rat anti-human IL-12 p70 specific antibody 20C2 (Cat. No. 555065) was paired with a biotinylated anti-IL-12 p40/p70 detection (Cat. No. 554660) to measure specifically human IL-12 p70 protein, using recombinant human IL-12 p70 (Cat. No. 554613), as the ELISA standard.

BD Pharmingen™ Biotin Mouse Anti-Human IL-12 (p40/p70) - Biotin - Clone C8.6 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified p40 Subunit of Human IL-12

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-IL12 (Biotin) (ABIN2689732)

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." dans: Immunity, Vol. 13, Issue 5, pp. 715-25, (2001) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." dans: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Détails sur IL12

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12 p40,p70

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome