TNF alpha anticorps (Biotin)

N° du produit ABIN2689928

Cet anticorps Human Monoclonal détecte spécifiquement TNF alpha dans ELISA. Il présente une réactivité envers Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour TNF alpha anticorps (Biotin) (ABIN2689928)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris, Rat

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est conjugé à/à la Biotin

Application: ELISA

Clone: 516D1A1

Détail du produit anti-TNF alpha anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The 516D1A1 Fab antibody reacts with mouse and rat tumor-necrosis factor (TNF, formerly known as TNF-α) proteins. The 516D1A1 Fab was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with recombinant mouse TNF protein. The biology and function of the TNF protein has been extensively reviewed (Hehlgans et al.) in the literature. The biotinylated 516D1A1 antibody is useful as a detection antibody for a sandwich ELISA that measures mouse and rat TNF protein levels. Purified Hamster Anti-Mouse/Rat TNF (Cat. No.557516) as capture antibody can be paired with biotinylated 516D1A1 as the detection antibody, using purified recombinant mouse TNF protein (Cat. No. 554589) or purified recombinant rat TNF protein (Cat. No. 555109) as standard. The biotinylated 516D1A1 antibody should be titrated between 0.25 - 2.0 μg/mL to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of purified mouse or rat TNF protein ranging from 31.25 pg/mL to 2,000 pg/mL are recommended for inclusion in each ELISA plate. Investigators may find methodology information to be useful in Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences (2003)-- Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. For this ELISA antibody pair, no cross-reactivity has been observed with the following: Rat IL-1α, IL-2, IL-4, IL-6, IL-10, IL-18, GM-CSF, IFN-γ, mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-18, M-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, sTNFRI, sTNFRII, RANTES, human TNF. Mouse TNF sandwich ELISA curve: The curve was generated by sandwich ELISA using the purified TN3-19.12 (Cat. No. 557516) as capture antibody, doubling dilutions of recombinant mouse TNF protein (Cat. No. 554589) and biotin 516D1A1 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.

BD Pharmingen™ Biotin Human anti-Mouse/Rat TNF - Biotin - Clone 516D1A1 - Isotype Human Ig, κ - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse TNF

Isotype: Ig Fraction, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-TNF alpha (Biotin) (ABIN2689928)

Hehlgans, Pfeffer: "The intriguing biology of the tumour necrosis factor/tumour necrosis factor receptor superfamily: players, rules and the games." dans: Immunology, Vol. 115, Issue 1, pp. 1-20, (2005) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome