DGKI anticorps
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- Antigène Voir toutes DGKI Anticorps
- DGKI (Diacylglycerol Kinase, iota (DGKI))
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Reactivité
- Humain, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DGKI est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of DGK-ι protein. (region surrounding Lys1023)
- Réactivité croisée (Details)
- Species reactivity (tested):Human, Rat.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Pureté
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product DGKI Anticorps primaire
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- Indications d'application
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- DO NOT FREEZE!
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C.
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- Antigène
- DGKI (Diacylglycerol Kinase, iota (DGKI))
- Autre désignation
- DAG Kinase iota (DGKI Produits)
- Synonymes
- anticorps DGK-IOTA, anticorps rDGKi-3, anticorps C130010K08Rik, anticorps diacylglycerol kinase iota, anticorps diacylglycerol kinase, iota, anticorps DGKI, anticorps dgki, anticorps LOC100560932, anticorps Dgki
- Sujet
- Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and - γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-´(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-Ω (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-^θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DGKI, Diacylglycerol kinase iota
- Poids moléculaire
- approx. 118 kDa
- ID gène
- 9162
- NCBI Accession
- NP_004708
- UniProt
- O75912
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