LIG4 anticorps

N° du produit ABIN272301

Cet anticorps anti-LIG4 Polyclonal Lapin (ABIN272301) détecte spécifiquement LIG4 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour LIG4 anticorps (ABIN272301)

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LIG4 anticorps

Specificité: This antibody detects endogenous levels of DNA Ligase IV protein. (region surrounding Gly619)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: DNA Ligase 4

Sujet: The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These proteins form a critical complex consisting of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligate accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination. V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate. Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resulting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation.Synonyms: DNA ligase IV, EC=6.5.1.1, LIG4, Polydeoxyribonucleotide synthase [ATP] 4

Poids moléculaire: 104 kDa

ID gène: 3981

NCBI Accession: NP_001091738

UniProt: P49917

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response