CD3 epsilon anticorps (Biotin)

N° du produit ABIN2739337

Cet anticorps anti-CD3 epsilon est un anticorps Hamster Monoclonal détectant CD3 epsilon dans FACS, WB, IP et ELISA. Adapté pour Souris.

Aperçu rapide pour CD3 epsilon anticorps (Biotin) (ABIN2739337)

Antigène: CD3 epsilon (CD3E)

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Clone: 145-2C11

Détail du produit anti-CD3 epsilon anticorps (Biotin)

Specificité: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

Purification: Protein G purified

Immunogène: Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated 5 minutes with 20 μL of 250 μg/mL Mouse IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.34 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated with 20^l of 0.5 mg/mL unlabeled anti-CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 4°C, avoid freeze-thaw cycles.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3E

Sujet: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

ID gène: 916

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway