TNF alpha anticorps

N° du produit ABIN2749097

Cet anticorps Souris Monoclonal détecte spécifiquement TNF alpha dans ELISA, ICC, IHC (fro) et ICFC. Il présente une réactivité envers Humain, Porc et Non-Human Primate et a été mentionné dans 4+ publications.

Aperçu rapide pour TNF alpha anticorps (ABIN2749097)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain, Porc, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: MAb11

Détail du produit anti-TNF alpha anticorps

Fonction: Anti-TNF alpha Purified

Specificité: The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).

Réactivité croisée (Details): Human, Non-Human Primates, Porcine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant human TNF-alpha

Isotype: IgG1 kappa

Information d'application

Indications d'application: ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1.
Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow cytometry: Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-TNF alpha (ABIN2749097)

Yan, Qing, Byers, Stadnyk, Al-Hertani, Bortolussi: "Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide." dans: Infection and immunity, Vol. 72, Issue 3, pp. 1223-9, (2004) (PubMed).

Attarbaschi, Willheim, Ramharter, Hofmann, Wahl, Winkler, Graninger, Winkler: "T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis)." dans: European cytokine network, Vol. 14, Issue 1, pp. 34-9, (2003) (PubMed).

Visser, Graffelman, Blauw, Haspels, Lentjes, de Kloet, Nagelkerken: "LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone." dans: Journal of neuroimmunology, Vol. 119, Issue 2, pp. 343-9, (2001) (PubMed).

Wahlström, Katchar, Wigzell, Olerup, Eklund, Grunewald: "Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis." dans: American journal of respiratory and critical care medicine, Vol. 163, Issue 1, pp. 115-21, (2001) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF alpha

Sujet: Tumor necrosis factor,TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.,TNFA, DIF, TNLG1F

ID gène: 7124

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome