RAD23B anticorps

N° du produit ABIN1944954

Détail du produit anti-RAD23B anticorps

Antigène: RAD23B (RAD23 Homolog B (RAD23B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD23B est non-conjugé

Application: Western Blotting (WB)

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C,-20 °C

Références pour anticorps anti-RAD23B (ABIN1944954)

Huang, Wang, Xu, Lu, Xu, Li, Zhou, Sha: "Expression of a novel RAD23B mRNA splice variant in the human testis." dans: Journal of andrology, Vol. 25, Issue 3, pp. 363-8, (2004) (PubMed).

Humphray, Oliver, Hunt, Plumb, Loveland, Howe, Andrews, Searle, Hunt, Scott, Jones, Ainscough, Almeida, Ambrose, Ashwell, Babbage, Babbage, Bagguley, Bailey, Banerjee, Barker, Barlow, Bates, Beasley et al.: "DNA sequence and analysis of human chromosome 9. ..." dans: Nature, Vol. 429, Issue 6990, pp. 369-74, (2004) (PubMed).

Masutani, Sugasawa, Yanagisawa, Sonoyama, Ui, Enomoto, Takio, Tanaka, van der Spek, Bootsma: "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23." dans: The EMBO journal, Vol. 13, Issue 8, pp. 1831-43, (1994) (PubMed).

Détails sur RAD23B

Antigène: RAD23B (RAD23 Homolog B (RAD23B))

Autre désignation: RAD23B (RAD23B Produits)

Synonymes: anticorps HHR23B, anticorps HR23B, anticorps P58, anticorps 0610007D13Rik, anticorps AV001138, anticorps mHR23B, anticorps p58, anticorps MGC107846, anticorps zgc:65951, anticorps RAD23 homolog B, nucleotide excision repair protein, anticorps UV excision repair protein RAD23 homolog B, anticorps RAD23 homolog B, nucleotide excision repair protein S homeolog, anticorps RAD23B, anticorps Rad23b, anticorps rd23b, anticorps rad23b, anticorps rad23b.S

Sujet: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER, it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

Poids moléculaire: 43171 Da

ID gène: 5887

UniProt: P54727

Pathways: Réparation de l'ADN