RAD9A anticorps (full length)

N° du produit ABIN363404

L’anticorps Lapin Polyclonal anti-RAD9A a été validé pour WB, IF et ICC. Il convient pour détecter RAD9A dans des échantillons de Humain.

Aperçu rapide pour RAD9A anticorps (full length) (ABIN363404)

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD9A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-RAD9A anticorps

Épitope: full length

Specificité: Reacts with human RAD9

Réactivité croisée (Details): Not tested in other species.

Purification: Antigen affinity purified

Immunogène: Fusion protein of full length human RAD9 protein

Information d'application

Indications d'application:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- ICC/IF : 1/500 - WB : 1/100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: , Sodium azide 0.1 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Date de péremption: 6 months

Détails sur RAD9A

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Autre désignation: RAD9

Sujet: Rad9 (cell cycle checkpoint control protein RAD9A or hRAD9) is an evolutionarily conserved protein which serves multiple functions for preserving genomic integrity, and besides its role as intra-S/G2 checkpoint , it also implicates in homologous recombination repair, base excision repair and mismatch repair pathways. PRKCD mediated Rad9 phosphorylation is essential for the assembly of 9-1-1 cell-cycle checkpoint response complex which is recruited to DNA lesion upon damage by RFC (RAD17-replication factor C) clamp loader complex. Thereafter, Rad9 acts as sliding clamp platform on DNA for multiple proteins involved in LP-BER (long-patch base excision repair). 9-1-1 complex stimulates DNA polymerase beta activity/stability, and is necessary for the recruitment of RHNO1 to sites of DNA-DSBs that occurs during S phase of cell cycle. Moreover, Rad9 exerts 3'->5' double stranded DNA exonuclease activity, and it transactivate p53 target genes such as p21 and has been shown to induce apoptosis through its interaction with Bcl-2/Bcl-xL.

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus