HSP27 anticorps (Center)

N° du produit ABIN2854951

Cet anticorps Lapin Polyclonal détecte spécifiquement HSP27 dans WB, IF, FACS, IP, IHC (p) et ICC. Il présente une réactivité envers Humain, Rat et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour HSP27 anticorps (Center) (ABIN2854951)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-HSP27 anticorps

Épitope: Center

Réactivité croisée: Macaque rhésus

Réactivité croisée (Details): Rhesus Monkey (100 %)

Attributs du produit: Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody

Purification: Affinity purified by Protein A.

Immunogène: Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*

Commentaires:

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 3.23 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-HSP27 (ABIN2854951)

Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." dans: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." dans: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSP27

Sujet: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle

Poids moléculaire: 23 kDa

ID gène: 3315

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling