POLR2B anticorps (N-Term)

N° du produit ABIN2855420

L’anticorps Lapin Polyclonal anti-POLR2B a été validé pour WB, ICC, IF et IHC (p). Il convient pour détecter POLR2B dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour POLR2B anticorps (N-Term) (ABIN2855420)

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2B est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLR2B anticorps

Épitope: N-Term

Réactivité croisée: Rat (Rattus), Macaque rhésus, Boeuf (Vache)

Réactivité croisée (Details): Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Attributs du produit: Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: H1299 , BCL-1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-POLR2B (ABIN2855420)

Jönsson, Ludvik Brattås, Gustafsson, Petri, Yudovich, Pircs, Verschuere, Madsen, Hansson, Larsson, Månsson, Meissner, Jakobsson: "Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors." dans: Nature communications, Vol. 10, Issue 1, pp. 3182, (2019) (PubMed).

Détails sur POLR2B

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Autre désignation: RPB2

Sujet: This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Cellular Localization: Nucleus

Poids moléculaire: 134 kDa

ID gène: 5431

Pathways: Regulatory RNA Pathways, Réparation de l'ADN