BTRC anticorps

N° du produit ABIN2855453

L’anticorps Lapin Polyclonal anti-BTRC a été validé pour WB et IHC (p). Il convient pour détecter BTRC dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour BTRC anticorps (ABIN2855453)

Antigène: BTRC (beta-Transducin Repeat Containing (BTRC))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BTRC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BTRC anticorps

Réactivité croisée: Humain, Rat

Attributs du produit: Rabbit polyclonal antibody to beta-TrCP (beta-transducin repeat containing)
beta-TrCP antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human beta-TrCP. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-BTRC (ABIN2855453)

Lin, Chang, Lee, Campbell, Wang, Shen, Chung, Chang, Chiu, Pan, Lin, Chu, Kung, Cheng, Chang: "REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling." dans: Oncotarget, Vol. 7, Issue 18, pp. 26137-51, (2018) (PubMed).

Détails sur BTRC

Antigène: BTRC (beta-Transducin Repeat Containing (BTRC))

Autre désignation: beta-transducin repeat containing E3 ubiquitin protein ligase

Sujet: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class, in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

Cellular Localization: Cytoplasm

Poids moléculaire: 69 kDa

ID gène: 8945

UniProt: Q9Y297

Pathways: Cycle Cellulaire, Signalisation Hedgehog