LIG4 anticorps

N° du produit ABIN2857063

Cet anticorps Lapin Polyclonal détecte spécifiquement LIG4 dans WB et IHC (p). Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour LIG4 anticorps (ABIN2857063)

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: KO Validated

Détail du produit anti-LIG4 anticorps

Réactivité croisée: Humain, Souris

Attributs du produit: Rabbit polyclonal antibody to DNA ligase IV (ligase IV, DNA, ATP-dependent)
DNA ligase IV antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human DNA ligase IV. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Molt-4

Validation: KO/KD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-LIG4 (ABIN2857063)

Ly, Teitz, Kim, Shoshani, Skaletsky, Fachinetti, Page, Cleveland: "Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining." dans: Nature cell biology, Vol. 19, Issue 1, pp. 68-75, (2017) (PubMed).

Zhu, Fisher, Bessho, Peng: "Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining." dans: Nucleic acids research, Vol. 45, Issue 18, pp. 10583-10594, (2017) (PubMed).

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: DNA ligase 4

Sujet: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Cellular Localization: Nucleus

Poids moléculaire: 104 kDa

ID gène: 3981

UniProt: P49917

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response