TNF alpha anticorps

N° du produit ABIN289560

Cité dans 3 publications. Cet anticorps anti-TNF alpha Polyclonal Lapin (ABIN289560) détecte spécifiquement TNF alpha dans WB, ELISA et Inhibition. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour TNF alpha anticorps (ABIN289560)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Détail du produit anti-TNF alpha anticorps

Purification: Purified

Immunogène: TNF alpha antibody was raised in rabbit using highly pure recombinant murine TNF-alpha as the immunogen.

Information d'application

Indications d'application: ELISA: 0.5-2 µg/mL, Inhibition: 0.04-0.05 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration 0.1 - 1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Références pour anticorps anti-TNF alpha (ABIN289560)

McCullough, McMullen, Poulsen, Kim, Medof, Nagy: "Anaphylatoxin Receptors C3aR and C5aR1 Are Important Factors That Influence the Impact of Ethanol on the Adipose Secretome." dans: Frontiers in immunology, Vol. 9, pp. 2133, (2019) (PubMed).

McCullough, McMullen, Das, Roychowdhury, Strainic, Medof, Nagy: "Differential contribution of complement receptor C5aR in myeloid and non-myeloid cells in chronic ethanol-induced liver injury in mice." dans: Molecular immunology, Vol. 75, pp. 122-32, (2017) (PubMed).

Zhuge, Cederbaum: "Inhibition of the mitochondrial permeability transition by cyclosporin A prevents pyrazole plus lipopolysaccharide-induced liver injury in mice." dans: Free radical biology & medicine, Vol. 46, Issue 3, pp. 406-13, (2009) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF alpha

Sujet: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

Poids moléculaire: 17.3 kDa (predicted detection band MW)

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome