IL-1 beta anticorps (Biotin)

N° du produit ABIN295086

Cet anticorps anti-IL-1 beta est un anticorps Chèvre Polyclonal détectant IL-1 beta dans WB et FACS. Adapté pour Chien.

Aperçu rapide pour IL-1 beta anticorps (Biotin) (ABIN295086)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-1 beta est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-IL-1 beta anticorps (Biotin)

Specificité: Recognizes canine Interleukin 1 beta (IL-1b).

Purification: Affinity purified

Immunogène: E. coli-derived recombinant canine IL-1beta.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: Flo, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Flow Cytometry and Western Blot. Western Blot: 0.1-0.2 μg/mL. Detection limit for recombinant canine IL-1beta is ~0.5 ng/lane under non-reducing and reducing conditions. Shows ~40 % cross-reactivity with recombinant feline IL-1beta, 10 % cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5 % cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta. Flow Cytometry: Dilute to 50 μg/mL. Use 10 μL of dilution to 1-2.5x10^5 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute, sterile 40-50 % glycerol, TBS (20 mM Trizma base, 150 mM sodium chloride), 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from PBS, BSA, labeled, biotin. Reconstitute, sterile 40-50 % glycerol, TBS (20 mM Trizma base, 150 mM sodium chloride), 0.1 % BSA.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur IL-1 beta

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL1B

Sujet: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

ID gène: 3553

UniProt: P01584

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome