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RAF1 anticorps (AA 657-668)

RAF1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Lapin, Chien, Cheval, Roussette (Chauve-souris), Hamster WB, IP Hôte: Mouton Polyclonal unconjugated
N° du produit ABIN295906
  • Antigène Voir toutes RAF1 Anticorps
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Épitope
    • 49
    • 43
    • 35
    • 34
    • 32
    • 27
    • 24
    • 23
    • 23
    • 22
    • 19
    • 18
    • 15
    • 13
    • 13
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 657-668
    Reactivité
    • 447
    • 230
    • 158
    • 13
    • 12
    • 12
    • 11
    • 8
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Singe, Lapin, Chien, Cheval, Roussette (Chauve-souris), Hamster
    Hôte
    • 479
    • 15
    • 2
    • 1
    Mouton
    Clonalité
    • 453
    • 44
    Polyclonal
    Conjugué
    • 202
    • 37
    • 28
    • 27
    • 20
    • 20
    • 20
    • 20
    • 20
    • 20
    • 17
    • 16
    • 10
    • 10
    • 10
    • 10
    • 10
    Cet anticorp RAF1 est non-conjugé
    Application
    • 297
    • 217
    • 105
    • 104
    • 89
    • 75
    • 49
    • 31
    • 30
    • 24
    • 16
    • 10
    • 10
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Specificité
    Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
    Homologie
    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
    Purification
    Protein A purified
    Immunogène
    12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product RAF1 Anticorps primaire
  • Indications d'application
    Approved: IP, WB

    Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 μg immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.
    Commentaires

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeat freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Autre désignation
    RAF1 / RAF (RAF1 Produits)
    Synonymes
    anticorps CRAF, anticorps NS5, anticorps Raf-1, anticorps c-Raf, anticorps c-raf, anticorps craf, anticorps raf1, anticorps RAF1, anticorps 6430402F14Rik, anticorps AA990557, anticorps BB129353, anticorps Craf1, anticorps D830050J10Rik, anticorps v-Raf, anticorps raf, anticorps cRaf, anticorps Raf-1 proto-oncogene, serine/threonine kinase, anticorps Raf-1 proto-oncogene, serine/threonine kinase a, anticorps v-raf-leukemia viral oncogene 1, anticorps Raf-1 proto-oncogene, serine/threonine kinase S homeolog, anticorps RAF1, anticorps Raf1, anticorps raf1a, anticorps raf1, anticorps raf1.S
    Sujet
    Name/Gene ID: RAF1
    Subfamily: Raf
    Family: Protein Kinase

    Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase
    ID gène
    5894
    UniProt
    P04049
    Pathways
    Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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