RAF1 anticorps (AA 657-668)
-
- Antigène Voir toutes RAF1 Anticorps
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
-
Épitope
- AA 657-668
-
Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Singe, Lapin, Chien, Cheval, Roussette (Chauve-souris), Hamster
-
Hôte
-
Mouton
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp RAF1 est non-conjugé
-
Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificité
- Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
- Purification
- Protein A purified
- Immunogène
-
12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product RAF1 Anticorps primaire
-
-
- Indications d'application
-
Approved: IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 μg immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro. - Commentaires
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
- Antigène
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- Autre désignation
- RAF1 / RAF (RAF1 Produits)
- Synonymes
- anticorps CRAF, anticorps NS5, anticorps Raf-1, anticorps c-Raf, anticorps c-raf, anticorps craf, anticorps raf1, anticorps RAF1, anticorps 6430402F14Rik, anticorps AA990557, anticorps BB129353, anticorps Craf1, anticorps D830050J10Rik, anticorps v-Raf, anticorps raf, anticorps cRaf, anticorps Raf-1 proto-oncogene, serine/threonine kinase, anticorps Raf-1 proto-oncogene, serine/threonine kinase a, anticorps v-raf-leukemia viral oncogene 1, anticorps Raf-1 proto-oncogene, serine/threonine kinase S homeolog, anticorps RAF1, anticorps Raf1, anticorps raf1a, anticorps raf1, anticorps raf1.S
- Sujet
-
Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase
Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase - ID gène
- 5894
- UniProt
- P04049
- Pathways
- Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
-