PKC beta anticorps (AA 607-671)

N° du produit ABIN296089

Cet anticorps Chèvre Polyclonal détecte spécifiquement PKC beta dans WB, ELISA et ICC. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour PKC beta anticorps (AA 607-671) (ABIN296089)

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC beta est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-PKC beta anticorps

Épitope: AA 607-671

Specificité: Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.

Purification: Affinity purified

Immunogène: E. coli-derived rhPKCb1 (aa607-671).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur PKC beta

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Autre désignation: PRKCB / PKC-Beta

Sujet: Name/Gene ID: PRKCB
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II

ID gène: 5579

UniProt: P05771

Pathways: Signalisation WNT, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling