PKC beta anticorps (AA 607-671)
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- Antigène Voir toutes PKC beta (PRKCB) Anticorps
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
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Épitope
- AA 607-671
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Reactivité
- Humain, Rat
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PKC beta est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Specificité
- Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
- Purification
- Affinity purified
- Immunogène
- E. coli-derived rhPKCb1 (aa607-671).
- Isotype
- IgG
- Top Product
- Discover our top product PRKCB Anticorps primaire
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- Indications d'application
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized.
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- Antigène
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
- Autre désignation
- PRKCB / PKC-Beta (PRKCB Produits)
- Synonymes
- anticorps PKC-beta, anticorps PKCB, anticorps PRKCB1, anticorps PRKCB2, anticorps A130082F03Rik, anticorps PKC-Beta, anticorps Pkcb, anticorps Prkcb1, anticorps Prkcb2, anticorps PKC-B, anticorps Prkcb, anticorps prkcb1, anticorps zgc:63591, anticorps PKC, anticorps PRKCB_tv2, anticorps Pkc53E, anticorps prkcb1l, anticorps zgc:64063, anticorps protein kinase C beta, anticorps protein kinase C, beta, anticorps protein kinase C, beta b, anticorps protein kinase C, beta a, anticorps PRKCB, anticorps Prkcb, anticorps prkcbb, anticorps prkcba
- Sujet
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Name/Gene ID: PRKCB
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II - ID gène
- 5579
- UniProt
- P05771
- Pathways
- Signalisation WNT, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
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