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Histone 3 (H3) (H3K4me3) anticorps Primary Antibody

H3 Reactivité: Humain, Souris, Rat ChIP, CUT&RUN, CUT&Tag, IF, IHC, IP, WB Hôte: Lapin Polyclonal
Pubmed (4)
N° du produit ABIN3023254
Plus shipping costs $45.00
local_shipping Destination: Etats-Unis
Envoi sous 13 à 14 jours ouvrables
  • Antigène
    Humain, Souris, Rat
    Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
    Affinity purification
    A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
  • Indications d'application
    Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:50-1:200
    For Research Use only
  • Format
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid freeze / thaw cycles
    -20 °C/-80 °C
  • Sun, Ermann, Niu, Yan, Yang, Shi, Zou: "Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts." dans: Bone research, Vol. 6, pp. 14, 2018 (PubMed). Méthode utilisée: Immunoprecipitation (IP)

    Yin, Jia, Miron, Long, Xu, Wei, Wu, Zhang, Li: "Setd7 and its contribution to Boron-induced bone regeneration in Boron-mesoporous bioactive glass scaffolds." dans: Acta biomaterialia, Vol. 73, pp. 522-530, 2018 (PubMed). Méthode utilisée: Immunohistochemistry (IHC)

    Liu, Wang, Gao, Liu, Liu: "iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 1, pp. 185-199, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

    Yu, Liu, Liu, Wang, Liu, Miao, Du, Yang: "Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes." dans: Scientific reports, Vol. 8, Issue 1, pp. 6132, 2018 (PubMed). Méthode utilisée: Immunofluorescence (IF)

  • Antigène
    Autre désignation
    Histone H3
    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
    Synonyms: HIST1H3J , H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3
    ID gène
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