ZAP70 anticorps (AA 1-254)

N° du produit ABIN3024068

Cet anticorps Souris Monoclonal détecte spécifiquement ZAP70 dans WB, FACS, IF et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour ZAP70 anticorps (AA 1-254) (ABIN3024068)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2F3-2

Détail du produit anti-ZAP70 anticorps

Épitope: AA 1-254

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the ZAP-70 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the ZAP-70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP-70

Sujet: ZAP70 is a 70  kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway