Smooth Muscle Actin anticorps (N-Term)
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- Antigène Voir toutes Smooth Muscle Actin (ACTA2) Anticorps
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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Épitope
- N-Term
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Reactivité
- Humain, Rat, Souris, Poulet, Boeuf (Vache), Lapin, Singe, Babouin, Cobaye, Chien, Cat, Porc, Chévre, Mouton
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Smooth Muscle Actin est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
- Clone
- 1A4
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product ACTA2 Anticorps primaire
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- Indications d'application
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Variations in protocols, secondaries and substrates may require the Smooth muscle actin antibody to be titered up or down for optimal performance.
1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).
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- Antigène
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- Autre désignation
- Smooth Muscle Actin (ACTA2 Produits)
- Synonymes
- anticorps AAT6, anticorps ACTSA, anticorps MYMY5, anticorps GAMMA-ACTIN, anticorps ACT, anticorps ACTA3, anticorps ACTE, anticorps ACTL3, anticorps ACTSG, anticorps Act-4, anticorps Act4, anticorps SMGA, anticorps 0610041G09Rik, anticorps Actvs, anticorps SMalphaA, anticorps a-SMA, anticorps alphaSMA, anticorps wu:fb63d03, anticorps ASMA, anticorps SMactin, anticorps a-sma, anticorps aat6, anticorps actsa, anticorps mymy5, anticorps sm-actin, anticorps ACTA2, anticorps actc1, anticorps ACT-4, anticorps asma, anticorps ACTGE, anticorps actin, alpha 2, smooth muscle, aorta, anticorps actin, gamma 2, smooth muscle, enteric, anticorps actin, alpha 2, smooth muscle, aorta L homeolog, anticorps ACTA2, anticorps ACTG2, anticorps Actg2, anticorps Acta2, anticorps acta2, anticorps acta2.L
- Sujet
- Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
- ID gène
- 59
- Pathways
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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