Cytochrome C anticorps (AA 1-80)

N° du produit ABIN3025122

Détail du produit anti-Cytochrome C anticorps

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Épitope: AA 1-80

Reactivité: Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Attributs du produit: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Purification: Protein G affinity chromatography

Immunogène: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.

Clone: 7H8-2C12

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome C (CYCS Produits)

Synonymes: anticorps CYC, anticorps HCS, anticorps THC4, anticorps cyct, anticorps cyc, anticorps hcs, anticorps CYCS, anticorps cytc, anticorps CYCSA, anticorps CG17903, anticorps Cyt C, anticorps Cyt c, anticorps Cyt-c2, anticorps CytC-2, anticorps Cytc, anticorps Cytc-p, anticorps DC4, anticorps DMc01, anticorps Dmel\\CG17903, anticorps cyt c, anticorps cyt c-p, anticorps cyt-c-p, anticorps cyt-c2, anticorps cyt.c, anticorps cytochrome c, anticorps dc4, anticorps pDMc01, anticorps wu:fk52b01, anticorps zgc:86706, anticorps ENSMUSG00000058927, anticorps cytochrome c, somatic, anticorps cytochrome c, somatic S homeolog, anticorps Cytochrome c proximal, anticorps cytochrome c, somatic b, anticorps cytochrome c, somatic pseudogene, anticorps cytochrome c-like, anticorps cytochrome c, anticorps CYCS, anticorps cycs.S, anticorps cycs, anticorps Cycs, anticorps Cyt-c-p, anticorps cycsb, anticorps Gm10053, anticorps LOC100724240, anticorps CYC, anticorps LOC100847700, anticorps LOC101107954, anticorps LOC106829744, anticorps LOC106992390, anticorps LOC102085712

Sujet: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity