ENO2/NSE anticorps (AA 416-433)
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- Antigène Voir toutes ENO2/NSE (ENO2) Anticorps
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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Épitope
- AA 416-433
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ENO2/NSE est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
- Clone
- NSEL1-1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ENO2 Anticorps primaire
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- Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10 mM Citrate Buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Autre désignation
- NSE (ENO2 Produits)
- Synonymes
- anticorps ENO2, anticorps DKFZp459B1817, anticorps NSE, anticorps AI837106, anticorps D6Ertd375e, anticorps Eno-2, anticorps RNEN3, anticorps eno3, anticorps wu:fc09h05, anticorps zgc:92418, anticorps enolase 2, anticorps enolase 2 (gamma, neuronal), anticorps enolase 2, gamma neuronal, anticorps enolase 2, gamma, neuronal, anticorps ENO2, anticorps Eno2, anticorps eno2
- Sujet
- Recognizes a protein of about 50 kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
- ID gène
- 2026
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