CD99 anticorps
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- Antigène Voir toutes CD99 Anticorps
- CD99
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD99 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aucune reactivité croisée
- Rat (Rattus)
- Purification
- Protein G affinity chromatography
- Immunogène
- Recombinant full-length human protein was used as the immunogen for the CD99 antibody.
- Clone
- MIC2-877
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD99 Anticorps primaire
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- Indications d'application
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Optimal dilution of the CD99 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- CD99
- Autre désignation
- CD99 (CD99 Produits)
- Synonymes
- anticorps 1110061M03Rik, anticorps 2410026K10Rik, anticorps D4, anticorps Pilr-l, anticorps pilr-1, anticorps HBA71, anticorps MIC2, anticorps MIC2X, anticorps MIC2Y, anticorps MSK5X, anticorps CD99 antigen, anticorps CD99 molecule (Xg blood group), anticorps CD99 molecule, anticorps Cd99, anticorps CD99
- Classe de substances
- Viral Protein
- Sujet
- Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. It is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.
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