TNFRSF8 anticorps

N° du produit ABIN3026172

Détail du produit anti-TNFRSF8 anticorps

Antigène: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNFRSF8 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: Human CD30 recombinant protein was used as the immungen for this antibody.

Clone: CD30-412

Isotype: IgG2b kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD30 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD30 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TNFRSF8

Antigène: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Autre désignation: CD30 (TNFRSF8 Produits)

Synonymes: anticorps CD30, anticorps D1S166E, anticorps Ki-1, anticorps Cd30, anticorps Ki, anticorps TNFRSF8, anticorps TNF receptor superfamily member 8, anticorps tumor necrosis factor receptor superfamily, member 8, anticorps TNFRSF8, anticorps Tnfrsf8

Sujet: The antibody recognizes a single chain glycoprotein of 105/120  kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90  kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120  kDa glycoprotein. In Hodgkin's disease, CD30 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This antibody distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the CD30 positive lymphomas lack the leukocyte common antigen (CD45).

ID gène: 943