HSP27 anticorps
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- Antigène Voir toutes HSP27 (HSPB1) Anticorps
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Reactivité
- Humain, Rat, Souris, Mouton, Poulet, Chimpanzé
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HSP27 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Attributs du produit
- This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced "p29" and "24K" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
- Purification
- Protein G affinity chromatography
- Immunogène
- Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
- Clone
- G3-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product HSPB1 Anticorps primaire
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- Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Autre désignation
- HSP27 (HSPB1 Produits)
- Synonymes
- anticorps CMT2F, anticorps HMN2B, anticorps HS.76067, anticorps HSP27, anticorps HSP28, anticorps Hsp25, anticorps SRP27, anticorps 27kDa, anticorps Hsp27, anticorps cb153, anticorps cb660, anticorps hsp1, anticorps hsp25, anticorps hsp27, anticorps id:ibd2821, anticorps sb:cb660, anticorps zgc:103437, anticorps HSPB1, anticorps cmt2f, anticorps hs.76067, anticorps hsp28, anticorps heat shock protein family B (small) member 1, anticorps heat shock protein 1, anticorps heat shock protein, alpha-crystallin-related, 1, anticorps heat shock protein family B (small) member 1 L homeolog, anticorps HSPB1, anticorps Hspb1, anticorps hspb1, anticorps hspb1.L
- Sujet
- This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced ""p29"" and ""24K"" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
- Pathways
- Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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