Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogène
An amino acid sequence from the C-terminal of human Calretinin (2EMNIQQLTNYRKSVMSLAEAGKLYRKDLE) was used as the immunogen for this Calretinin antibody.
CALB2
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
The stated application concentrations are suggested starting amounts. Titration of the Calretinin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Stock
-20 °C
Stockage commentaire
After reconstitution, the Calretinin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Calretinin, also known as 29 kDa calbindin, is a vitamin D-dependent calcium-binding protein involved in calcium signaling. In humans, the protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Calretinin is mapped to 16q22.2. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers.