EIF3F anticorps (AA 114-125)

N° du produit ABIN303092

Détail du produit anti-EIF3F anticorps

Antigène: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Épitope: AA 114-125

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Poulet, Drosophila melanogaster, Poisson

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF3F est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: Amino acids 114-125 of Human eIF3f protein.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Chimpanzee, Chicken, Drosophila, Fish , Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: KLH Synthetic peptide corresponding to amino acids 114-125 of Human eIF3f protein.

Information d'application

Indications d'application: Western Blot (1/200-1/2000). ELISA (1/10000-1/40000). Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This EIF3 F antibody was validated for use in Immunohistochemistry on a panel of 23 ormalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.

Détails sur EIF3F

Antigène: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Autre désignation: EIF3F / EIF3S5 (EIF3F Produits)

Synonymes: anticorps AO090005001590, anticorps Dmel\CG9769, anticorps eIF3-S5, anticorps eif3f, anticorps LOC733116, anticorps eIF3f, anticorps EIF3S5, anticorps eIF3-p47, anticorps 0610037M02Rik, anticorps AA409853, anticorps Eif3s5, anticorps eukaryotic translation initiation factor 3 subunit F, anticorps hypothetical protein, anticorps CG9769 gene product from transcript CG9769-RA, anticorps eukaryotic translation initiation factor 3 subunit 5, anticorps eukaryotic translation initiation factor 3, subunit F, anticorps Eukaryotic translation initiation factor 3 subunit F, anticorps eif3f, anticorps ANI_1_120074, anticorps AOR_1_2780174, anticorps PTRG_03897, anticorps PAAG_00376, anticorps MCYG_08502, anticorps MGYG_08653, anticorps PGTG_04587, anticorps CG9769, anticorps LOC733116, anticorps EIF3F, anticorps Eif3f, anticorps eif-3.F

Sujet: Eukaryotic translation initiation factor 3 subunit F also known as eIF3f, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.Synonyms: Eukaryotic translation initiation factor 3 subunit 5, Eukaryotic translation initiation factor 3 subunit F, eIF-3-epsilon, eIF3 p47

ID gène: 8665

NCBI Accession: NP_003745

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization