PAD4 anticorps (Middle Region)

N° du produit ABIN3032172

Détail du produit anti-PAD4 anticorps

Antigène: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Épitope: Middle Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAD4 est non-conjugé

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogène: An amino acid sequence from the middle region of human PAD4 (ASPRSCYKLFQEQQNEGHGEA) was used as the immunogen for this PAD4 antibody.

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the PAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the PAD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur PAD4

Antigène: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Autre désignation: PAD4 (PADI4 Produits)

Synonymes: anticorps PAD, anticorps PAD4, anticorps PADI5, anticorps PDI4, anticorps PDI5, anticorps Pad4, anticorps Pdi4, anticorps peptidyl arginine deiminase 4, anticorps peptidyl arginine deiminase, type IV, anticorps PADI4, anticorps Padi4

Sujet: Peptidylarginine Deiminase, Type IV is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that the protein specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PAD4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PAD4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that the protein could repress hormone receptor-mediated gene induction. It was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role.

UniProt: Q9UM07