RFC1 anticorps (N-Term)

N° du produit ABIN3043075

Détail du produit anti-RFC1 anticorps

Antigène: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Épitope: AA 46-64, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.

Séquence: NSSRKEDDFK QKQPSKKKR

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
Gene Name: replication factor C(activator 1) 1, 145 kDa
Protein Name: Replication factor C subunit 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1(46-64aa NSSRKEDDFKQKQPSKKKR).

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur RFC1

Antigène: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Autre désignation: RFC1 (RFC1 Produits)

Synonymes: anticorps A1, anticorps MHCBFB, anticorps PO-GA, anticorps RECC1, anticorps RFC, anticorps RFC140, anticorps AtRFC1, anticorps replication factor C 1, anticorps replication factor C1, anticorps 140kDa, anticorps Alp145, anticorps Recc1, anticorps Replication factor C1, anticorps si:dkey-170e5.1, anticorps zgc:162875, anticorps replication factor C subunit 1, anticorps replication factor C1, anticorps replication factor C (activator 1) 1, anticorps DNA replication factor C complex subunit Rfc1, anticorps RFC1, anticorps Rfc1, anticorps rfc1

Sujet: RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.

Synonyms: A1 140 kDa subunit antibody|A1 antibody|A1 P145 Activator 1 large subunit antibody|Activator 1 140 kDa subunit antibody|Activator 1 large subunit antibody|Activator 1 subunit 1 antibody|DNA binding Protein PO GA antibody|DNA-binding protein PO-GA antibody|MHC binding factor beta antibody|MHCBFB antibody|PO GA antibody|RECC1 antibody|Replication factor C(activator 1) 1, 145 kDa antibody|Replication factor C 140 kDa subunit antibody|Replication factor C antibody|Replication factor C large subunit antibody|Replication factor C subunit 1 antibody|Replication factor C1 antibody|RF-C 140 kDa subunit antibody|RFC antibody|RFC1 antibody|RFC1_HUMAN antibody|RFC140 antibody|RFC140 Replication Factor C 140 kDa subunit antibody

UniProt: P35251

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport