PARP1 anticorps (AA 670-858)

N° du produit ABIN3043378

Cet anticorps Lapin Polyclonal détecte spécifiquement PARP1 dans WB, IHC, ICC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 2+ publications.

Aperçu rapide pour PARP1 anticorps (AA 670-858) (ABIN3043378)

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-PARP1 anticorps

Épitope: AA 670-858

Fonction: Anti-PARP/PARP1 Antibody

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: Human PARP shares 94%,95% amino acid (aa) sequence identity with mouse,rat PARP,respectively.

Attributs du produit: Anti-PARP/PARP1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, RatImmunocytochemistry , 2 μg/mL, Human, RatImmunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PARP1 (ABIN3043378)

Liu, Yang, Mu, Fu: "Epigallocatechin-3-Gallate Ameliorates Glucocorticoid-Induced Osteoporosis of Rats in Vivo and in Vitro." dans: Frontiers in pharmacology, Vol. 9, pp. 447, (2018) (PubMed).

Li, Li, Xu, Guo, Du: "Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia." dans: International journal of molecular sciences, Vol. 11, Issue 11, pp. 4580-90, (2010) (PubMed).

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: PARP1

Sujet:

Background: Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Gene Full Name: poly(ADP-ribose) polymerase 1

Sequence Similarities: Contains 1 BRCT domain.

Poids moléculaire: 120 kDa

ID gène: 142

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location