PDK4 anticorps (N-Term)

N° du produit ABIN3043461

Cet anticorps anti-PDK4 est un anticorps Lapin Polyclonal détectant PDK4 dans WB. Adapté pour Humain et Rat.

Aperçu rapide pour PDK4 anticorps (N-Term) (ABIN3043461)

Antigène: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDK4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PDK4 anticorps

Épitope: AA 91-125, N-Term

Fonction: Anti-PDK4 Antibody Picoband®

Séquence: WYIQSLMDLV EFHEKSPDDQ KALSDFVDTL IKVRN

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PDK4 Antibody Picoband® (ABIN3043461). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PDK4, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: PDK4 pyruvate dehydrogenase kinase, isozyme 4". 2. Andrews MT, Squire TL, Bowen CM, Rollins MB (Jul 1998). "Low-temperature carbon utilization is regulated by novel gene activity in the heart of a hibernating mammal". Proceedings of the National Academy of Sciences of the United States of America 95 (14): 8392-7. 3. Gudi R, Bowker-Kinley MM, Kedishvili NY, Zhao Y, Popov KM (Dec 1995). "Diversity of the pyruvate dehydrogenase kinase gene family in humans".The Journal of Biological Chemistry 270 (48): 28989-94.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PDK4

Antigène: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Autre désignation: PDK4

Sujet:

Synonyms: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial,2.7.11.2,Pyruvate dehydrogenase kinase isoform 4,PDK4,PDHK4,

Tissue Specificity: Ubiquitous, highest levels of expression in heart and skeletal muscle. .

Background: Pyruvate dehydrogenase lipoamide kinase isozyme 4, mitochondrial is an enzyme that in humans is encoded by the PDK4 gene. This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. In addition, PDK4 is increased in hibernation and helps to decrease metabolism and conserve glucose by decreasing its conversion to acetyl-CoA, which enters the citric acid cycle and is converted to ATP.

Poids moléculaire: 46 kDa

ID gène: 5166

UniProt: Q16654

Pathways: Signalisation PI3K-Akt, Signalisation Hedgehog, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg