PKC epsilon anticorps (AA 53-236)

N° du produit ABIN3043908

Cet anticorps anti-PKC epsilon est un anticorps Lapin Polyclonal détectant PKC epsilon dans WB et IHC. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour PKC epsilon anticorps (AA 53-236) (ABIN3043908)

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC epsilon est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PKC epsilon anticorps

Épitope: AA 53-236

Fonction: Anti-PKC epsilon/PRKCE Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PKC epsilon/PRKCE Antibody Picoband® (ABIN3043908). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Basta P, Strickland MB, Holmes W, Loomis CR, Ballas LM, Burns DJ (Nov 1992). "Sequence and expression of human protein kinase C-epsilon". Biochim Biophys Acta 1132 (2): 154-60. 2. Knauf, J. A., Elisei, R., Mochly-Rosen, D., Liron, T., Chen, X.-N., Gonsky, R., Korenberg, J. R., Fagin, J. A. Involvement of protein kinase C-epsilon (PKC-epsilon) in thyroid cell death: a truncated chimeric PKC-epsilon cloned from a thyroid cancer cell line protects thyroid cells from apoptosis. J. Biol. Chem. 274: 23414-23425, 1999. 3. Premkumar, L. S., Ahern, G. P. Induction of vanilloid receptor channel activity by protein kinase C. Nature 408: 985-990, 2000.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-PKC epsilon (ABIN3043908)

Chen, Wang, Zhang, Wan: "Low expression of PKCα and high expression of KRAS predict poor prognosis in patients with colorectal cancer." dans: Oncology letters, Vol. 12, Issue 3, pp. 1655-1660, (2016) (PubMed).

Détails sur PKC epsilon

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Autre désignation: PRKCE

Sujet:

Synonyms: Protein kinase C epsilon type,2.7.11.13,nPKC-epsilon,PRKCE,PKCE,

Background: Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.

Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Poids moléculaire: 88 kDa

ID gène: 5581

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling