SQSTM1 anticorps (N-Term)

N° du produit ABIN3043937

L’anticorps Lapin Polyclonal anti-SQSTM1 a été validé pour WB, IHC, IF et ICC. Il convient pour détecter SQSTM1 dans des échantillons de Humain, Souris et Rat. Il y a 5+ publications disponibles.

Aperçu rapide pour SQSTM1 anticorps (N-Term) (ABIN3043937)

Antigène: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SQSTM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-SQSTM1 anticorps

Épitope: AA 69-96, N-Term

Fonction: Anti-SQSTM1/p62 Antibody Picoband®

Séquence: DEDGDLVAFS SDEELTMAMS YVKDDIFR

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-SQSTM1/p62 Antibody Picoband® (ABIN3043937). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 2 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry, 0.5-1 μg/mL, Human1. Hocking, L. J., Lucas, G. J. A., Daroszewska, A., Mangion, J., Olavesen, M., Cundy, T., Nicholson, G. C., Ward, L., Bennett, S. T., Wuyts, W., Van Hul, W., Ralston, S. H. Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease. Hum. Molec. Genet. 11: 2735-2739, 2002. 2. Joung, I., Strominger, J. L., Shin, J. Molecular cloning of a phosphotyrosine-independent ligand of the p56-lck SH2 domain. Proc. Nat. Acad. Sci. 93: 5991-5995, 1996. 3. Park, I., Chung, J., Walsh, C. T., Yun, Y., Strominger, J. L., Shin, J. Phosphotyrosine-independent binding of a 62- kDa protein to the src homology 2 (SH2) domain of p56-lck and its regulation by phosphorylation of ser-59 in the lck unique N-terminal region. Proc. Nat. Acad. Sci. 92: 12338-12342, 1995.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-SQSTM1 (ABIN3043937)

Yu, Li, Wang, He, Ding, Zhang, Yu, Shi, Cui, Wang, Wang, Sun, Zhang, Du, Zhu: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." dans: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).

Song, Hu, Li, Zheng, Wang, Guo, Shi, Liu: "Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication." dans: Archives of virology, Vol. 163, Issue 1, pp. 135-144, (2018) (PubMed).

Jeong, He, Nohara, Park, Shin, Kim, Shimomura, Koike, Yoo, Chen: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." dans: Scientific reports, Vol. 5, pp. 12801, (2017) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." dans: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Li, Jen, Wu, Lee, Fang, Quigley, Lee, Wang, Zhou, Vergnes, Chen, Li, Reue, Ann, Hsiai: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." dans: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, (2016) (PubMed).

Détails sur SQSTM1

Antigène: SQSTM1 (Sequestosome 1 (SQSTM1))

Autre désignation: SQSTM1

Sujet:

Synonyms: Sequestosome-1,EBI3-associated protein of 60 kDa,EBIAP,p60,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,Ubiquitin-binding protein p62,SQSTM1,ORCA, OSIL,

Tissue Specificity: Ubiquitously expressed. .

Background: SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Sequence Similarities: Contains 1 PB1 domain.

Poids moléculaire: 62 kDa

ID gène: 8878

UniProt: Q13501

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Autophagy