TP53BP1 anticorps (C-Term)

N° du produit ABIN3044038

Cet anticorps Lapin Polyclonal détecte spécifiquement TP53BP1 dans IHC, WB et ICC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TP53BP1 anticorps (C-Term) (ABIN3044038)

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TP53BP1 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Détail du produit anti-TP53BP1 anticorps

Épitope: C-Term

Fonction: Anti-53BP1/TP53BP1 Antibody

Séquence: ERIGFKQHPK YKHDYVSH

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: identical to the related mouse sequence.

Attributs du produit: Anti-53BP1/TP53BP1 Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1, identical to the related mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry , 0.5-1 μg/mL, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur TP53BP1

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Autre désignation: TP53BP1

Sujet:

Background: TP53BP1 (Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al. (1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al. (1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al. (1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21 (CDKN1A). Wang et al. (2002) used small interfering RNA ed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Gene Full Name: tumor protein p53 binding protein 1

Sequence Similarities: Contains 2 BRCT domains.

Poids moléculaire: 450 kDa

ID gène: 7158

UniProt: Q12888

Pathways: Réparation de l'ADN